Functional importance of individual rRNA 2'-O-ribose methylations revealed by high-resolution phenotyping
نویسندگان
چکیده
منابع مشابه
Detailed Mitochondrial Phenotyping by High Resolution Metabolomics
Mitochondrial phenotype is complex and difficult to define at the level of individual cell types. Newer metabolic profiling methods provide information on dozens of metabolic pathways from a relatively small sample. This pilot study used "top-down" metabolic profiling to determine the spectrum of metabolites present in liver mitochondria. High resolution mass spectral analyses and multivariate ...
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RlmM (YgdE) catalyzes the S-adenosyl methionine (AdoMet)-dependent 2'O methylation of C2498 in 23S ribosomal RNA (rRNA) of Escherichia coli. Previous experiments have shown that RlmM is active on 23S rRNA from an RlmM knockout strain but not on mature 50S subunits from the same strain. Here, we demonstrate RlmM methyltransferase (MTase) activity on in vitro transcribed 23S rRNA and its domain V...
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A single, unique structure of protein determined by X-ray crystal is often used for studies in structure–function relationships. However, the protein is naturally dynamic and fluctuation in solution, the single unique structure is insufficient to illuminate the dynamic character and “personalities” [1,2]. Other than a theoretical calculation approach, such as molecular dynamics simulation is ab...
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ژورنال
عنوان ژورنال: RNA
سال: 2008
ISSN: 1355-8382
DOI: 10.1261/rna.845808